Indian DNA Test Options
in Native American Genealogy

By itself, no Indian DNA test will grant you admission to a particular tribe. Depending on your situation, however, DNA testing may be just what you need to define your family’s biological relationship to Native Americans.

There are many alternatives for an Indian DNA test. To choose the correct one for your situation, please review the following:

You think a particular MALE ancestor may have been a Native American

Since the ancestor in question was male, you can use a Y-DNA test of a male in that man’s direct paternal line, i.e. any son, or any sons of those sons, or any of their sons etc. You want to know the paternal haplogroup.

If the haplogroup is C or Q, then it’s highly likely that this ancestor is descended from Native Americans who were here before European contact. If the haplogroup subgroup is C3b or Q1a3a, then you have absolute confirmation.

I recommend Family Tree DNA for Y-DNA testing. To see why, read my FTDNA Review. Testing 12 markers with them is sufficient to detect the Native American haplogroups. If you’re interested, they offer additional tests to further refine your haplogroup.

NOTE: If your haplogroup is not C or Q, this does not rule out Native American ancestry in another line. For example, many men of eastern U.S. tribes, such as Cherokee, have a European haplogroup like R1b. That’s because there was a lot of intermingling with the early settlers from Europe. So your Indian ancestor with a European haplogroup could be culturally Indian and genetically Indian through a female line.

You think a particular FEMALE ancestor may have been a Native American

Since the ancestor in question was female, she did not have a Y chromosome to pass on. She did pass on her mitochondrial DNA. So you need an mtDNA test of a man or woman in that ancestor’s direct maternal line, i.e. any child of hers, or any child of her daughters, or any child of her daughter’s daughters etc. You want to know the maternal haplogroup.

Maternal haplogroups that indicate Native American heritage are A, B, C, D, and sometimes X. Unlike the paternal line, there is no subgroup that can provide absolute proof of Indian heritage. But if that ancestor’s family did not immigrate from elsewhere, you can be quite sure of the findings.

A basic mtDNA test of the HVR-1 region is sufficient to determine the base haplogroup. I recommend Family Tree DNA for mtDNA testing.

You think you’re part Native American but can’t identify a specific ancestor

In this case, your first choice for an Indian DNA test would be one of the new DNA tests that check over half a million autosomal markers. These tests looks at DNA inherited from ALL your ancestors. They are not as precise as the tests mentioned above. But they are much broader tests, because they are not limited to paternal or maternal lines.

The two tests are the Ancestry Edition at 23andMe. and the Family Finder product at Family Tree DNA. The 23andMe test reports your relative admixtures of European, Asian, and African ancestry. Since Native Americans originally migrated from Asia via Alaska, they are included in the Asian total.

If your Asian percentage is zero, then you can probably rule out Native American Ancestry within the last few generations. But if your Asian percentage is significant and your family has no recent ancestors from Asian countries, then it is reasonable to assume that your family tree includes some native Americans.

At the time I'm writing this page, the Family Finder product is only available to Beta testers at Family Tree DNA. The tool for determining your admixture is still under development. But early indications suggest it may provide an even better admixture report.

You are part Native American and want to expand your family tree

If you are male with one of the Native American paternal haplogroups, I recommend you get a 67-marker Y-DNA test at Family Tree DNA.

You will be able to identify and contact men with whom you share a common ancestor on the paternal line. One or more of these matches may have the genealogical information you need.

Males and females with one of the Native American maternal haplogroups should order the mtDNA Plus or the mtDNA Full Sequence test from Family Tree DNA. This may lead you to people who share a common female ancestor and know more about that line.

NOTE: With mtDNA testing the timeframe of common ancestors may be too far back to provide useful genealogical information.

In either case you should also join one or more of the Native American or relevant geographic group projects at Family Tree DNA.

If you took the Ancestry Edition test at 23andMe (or the Complete Edition that adds medical implications), be sure to check their Relative Finder.
If you do happen to have any relatives in the database, this tool can estimate the degree of relationship and allow you to request contact with them. But without further analysis, it can't tell you which branch of your family has the common ancestor. It may not be from the Indian branch.

As I write this, I am Beta testing the similar Family Finder product at Family Tree DNA. Since customers of that company are more likely to be genealogists, any contacts you make will probably be more productive. To learn from my ongoing experience with these two products I suggest you scroll back up this page and subscribe to my web site using the buttons at the left.

You are part Native American and have the funds to dig even deeper

The specific tests mentioned above are all tests that I have taken myself. So I’m confident in recommending them. I do not, however, have any Native American ancestors. Those who do may want to consider two additional tests that may have value as an Indian DNA test.

Ancestry by DNA

The Ancestry by DNA test only looks at 350 autosomal markers. But those are "Ancestry Informative Markers" where the frequency of the marker values (alleles) varies in different populations.

Their admixture report attempts to separate “Asian” ancestry into Indigenous American and East Asian components. This could be more useful for an Indian DNA test. But the population data is from relatively small samples. So the accuracy can be severely limited by statistical noise.

NOTE: Those who have been around DNA testing for awhile will recognize this as the old DNA Print test licensed to a different company.

DNA Tribes

Another possibility for an Indian DNA test is DNA Tribes. They use a set of 21 autosomal markers, including the CODIS markers used by police in forensic investigations. But instead of looking for differences that can uniquely define one individual, they look for similarities. They compare your DNA to a global database of more than 1,000 ethnic populations.

Depending on your matches, this Indian DNA test may be able to report more specific tribes like Apache and Navaho. But as with the prior test, it is currently impossible to get DNA data on large numbers of ethically pure individuals. So the statistical confidence is low. And you should only consider these results as a possible clue to your Native American heritage.

Your Best Source for Extra Help

I recommend Roberta Estes as an expert on Native American history and Indian DNA Testing. Read her excellent white paper. Through her web site, DNA Explain, she can devise a customized Indian DNA test plan to best achieve your goals. Once you get your results, she offers various analysis packages to help you get the most information from your data.

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